Formulation of Fish Egg Snacks Substituting Tuna (Euthynnus affinis) Protein Concentrate: Sensory, Proximate, and Amino Acid Characteristics
DOI:
https://doi.org/10.47134/inhis.v5i1.161Keywords:
tuna fish, protein, concentrate, amino acidsAbstract
Background: Tuna is a fish known for its high protein content and is widely consumed by the community. “Fish egg snacks” are popular among people from diverse backgrounds. Purpose: This study was to determine the acceptability, nutritional content, and amino acid profile of “fish egg snack” formulations with tuna protein concentrate substitution. Methods: This study used an experimental design. The subjects used in the organoleptic testing were 25 semi-trained panelists. The stages of this study included the production of tuna protein concentrate and the production of fish egg snack. The analyses conducted included organoleptic analysis, nutritional content analysis, and amino acid profile analysis. The data were analyzed descriptively. Results: F4 was the best formula based on the De Garmo assessment of organoleptic testing and protein content. F4 was found to have a total energy of 496.86 kcal, ash content of 2.35%, moisture content of 2.51%, carbohydrates of 50.97%, and total fat content of 23.26%. In addition, the most abundant amino acid found in F4 was L-glutamic acid. Conclusion: F4 is the best fish egg snack formulation, and L-glutamic acid is the most abundant amino acid found in F4.
References
Ajomiwe, N., Boland, M., Phongthai, S., Bagiyal, M., Singh, J., & Kaur, L. (2024). and Bioavailability for Optimal Health. Foodborne Parasites in the Food Supply Web: Occurrence and Control, 13(11), 1–15.
Aloka, B., Olum, S., & Ongeng, D. (2025). Evaluation of the nutritional quality of food composites developed from local ingredients to target the needs of persons experiencing nodding syndrome in Northern Uganda. Scientific Reports, 15(1), 1–14. https://doi.org/10.1038/s41598-025-25483-6
Asfar, M., Maksum, F., Laga, A., Tawali, A. B., Mahendradatta, M., Akil, A. W., Paena, M., & Lestari, D. (2025). Nutritional characterization and functional properties of milkfish (Chanos chanos) protein concentrate and whole fish powder as protein and calcium ingredient products. Food Chemistry Advances, 9(September), 101114. https://doi.org/10.1016/j.focha.2025.101114
Asriani, Santoso, J., & Listyarini, S. (2021). Jurnal Kemaritiman : Indonesian Journal of Maritime Konsentrat Protein Ikan Lele Dumbo ( Clarias gariepenus ) Afkir. Indonesian Journal of Maritime, 2(2), 97–104. https://ejournal.upi.edu/index.php/kemaritiman/article/view/35484
Breemer, R., Lahagu, O., & Palijama, S. (2023). Pengaruh Konsentrasi Tepung Ikan Cakalang ( Katsuwonus pelamis ) dan Lama Pengeringan Terhadap Karakteristik Kimia Tortilla Chips Ubi Jalar Ungu ( Ipomoea batatas L ) The Effect of Concentration of Skipjack Tuna ( Katsuwonus p. Jurnal Agrosilvopasture-Tech, 2(2), 548–554.
Pratiwi, I. G. (2023). Studi Literatur: Intervensi Spesifik Penanganan Stunting. Indonesian Health Issue, 2(1), 29–37. https://doi.org/10.47134/inhis.v2i1.43
Connolly, G., Hudson, J. L., Bergia, R. E., Davis, E. M., Hartman, A. S., Zhu, W., Carroll, C. C., & Campbell, W. W. (2023). Effects of Consuming Ounce-Equivalent Portions of Animal- vs. Plant-Based Protein Foods, as Defined by the Dietary Guidelines for Americans on Essential Amino Acids Bioavailability in Young and Older Adults: Two Cross-Over Randomized Controlled Trials. Nutrients, 15(13). https://doi.org/10.3390/nu15132870
Damongilala, L. J. (2021). Kandungan Gizi Pangan Ikani. Bandung: CV Patra Media Grafindo.
Fikriyah, Y. U., & Reni Silvia Nasution. (2021). Analisis Kadar Air Dan Kadar Abu Pada Teh Hitam Yang Dijual Di Pasaran Dengan Menggunakan Metode Gravimetri. Ar-Raniry Chemistry Journal, 3(2), 50–54.
Jamaluddin, Hajra, Lisnawati, N. M. Y., Putri, G. N., Pitriani, & Bohari. (2023). Formulasi Pembuatan Biskuit Crackers Berbasis Tepung Ikan Sidat dan Daun Kelor. Jurnal Ilmiah Kesmas-IJ, 22(1), 32–38.
Kumoro, A. C., Wardhani, D. H., Kusworo, T. D., Djaeni, M., Ping, T. C., & Ma’rifat Fajar Azis, Y. (2022). Fish protein concentrate for human consumption: A review of its preparation by solvent extraction methods and potential for food applications. Annals of Agricultural Sciences, 67(1), 42–59. https://doi.org/10.1016/j.aoas.2022.04.003
Loveday, S. M. (2023). Protein digestion and absorption: the influence of food processing. Nutrition Research Reviews, 36(2), 544–559. https://doi.org/10.1017/S0954422422000245
Lujuo, J. E., Mkupasi, E. M., Sciences, B., Lamtane, H. A., & Sciences, R. (2025). Proximate Composition of Frozen Tuna and Tuna-like Fillets Marketed in Tanga and Mtwara along the Tanzanian Coastline Corresponding Author : Proximate Composition of Frozen Tuna and Tuna-like Fillets Marketed in Tanga and Mtwara along the Tanzanian Coastl. 1–14.
Mardiyah, U., Jamil, S., Muqsith, A., & Rodiyah, A. (2022). Analisis Sensori dan Nilai Gizi Snack Bar Substitusi Tepung Ikan Teri (Stolephorus sp. Jurnal Ilmu Perikanan, 13(2), 155–161.
Mosikyan, S., Dolan, R., Corsi, A. M., & Bastian, S. (2024). A systematic literature review and future research agenda to study consumer acceptance of novel foods and beverages. Appetite, 203(May), 107655. https://doi.org/10.1016/j.appet.2024.107655
Mustofa, B.K. (2013). Studi Eksperimen Pembuatan Telur gabus Dari Bahan Dasar "Pati Garut”. Universitas Negeri Semarang.
Amalia, N.R., Asri S.N., & Nikmawatisusanti, Y. Y. (2023). Pengaruh Subtitusi Tepung Ikan Layang (decapterus sp) Terhadap Formulasi Mie Kering. Jurnal Ilmiah Multidisiplin, 2(1), 13–22. https://doi.org/10.1080/15411796.2011.585906
Ramadhani, F., & Murtini, E. S. (2017). Pengaruh jenis tepung dan penambahan perenyah terhadap karakteristik fisikokimia dan organoleptik kue telur gabus keju. Jurnal Pangan Dan Agroindustri, 5(1), 38–47.
Ramadhani, T., Anggo, A. D., & Purnamayati, L. (2022). Pengaruh Fortifikasi Konsentrat Protein Ikan Kembung (Rastrelliger sp.) terhadap Kualitas Keripik. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 17(1), 53. https://doi.org/10.15578/jpbkp.v17i1.806
Rieuwpassa, F. J., & Karimela, E. J. (2024). Analisis Standar Mutu dan Asam Amino Konsentrat Protein Ikan Sunglir (Elagatis bipinnulatus). Jurnal Teknologi Pangan, 7(1), 1–5. https://doi.org/10.14710/jtp.2023.22419
Sabarella, Komalasari WB, Manurung M, Saida MDN, Seran K, & Supriyati Y. (2024). Buletin Konsumsi Pangan. Pus Data Dan Sist Inf Pertan Sekr Jenderal, Kementeri Pertan., 1–82.
Salampessy, R. B. S., Irianto, H. E., & Alifah, R. N. (2023). Mixture Design Application on the Development of Mackerel Tuna (Euthynnus affinis) Snack Bars as Healthy Snacks. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 400–413. https://doi.org/10.17844/jphpi.v26i3.43748
Setyarini, D., Bustami, & Joko, S. (2024). Karakteristik kimia dan sifat fungsional konsentrat protein ikan (KPI) dan tepung tulang dari ikan lele: Chemical characteristics and functional properties of fish protein concentrate (FPC) and catfish bone meal. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(6), 459–473. https://journal.ipb.ac.id/jphpi/article/view/50064
Simeunović, J., Miljanić, J., Kokić, B., Perović, L., Jovančević, J., Glušac, J., & Kojić, J. (2025). Protein Fortification of Millet-Based Gluten-Free Snacks Designed for 3D Printing. Foods, 14(24), 1–24. https://doi.org/10.3390/foods14244308
Verrenisa Melati Haryani, Dittasari Putriana, R. W. H. (2023). Asupan Protein Hewani Berhubungan Dengan Stunting Pada Balita Di Wilayah Kerja Puskesmas Minggir. Amerta Nutr, 7(2), 139–146. https://doi.org/10.20473/amnt.v7i2SP.2023.13
Warkey, A. A., Leiwakabessy, J., & Matrutty, T. E. A. A. (2023). Fortifikasi Konsentrat Protein Ikan Tuna Fortification Of Tuna Fish Protein Concentrate In The Making Of Finger Stick. Jurnal Teknologi Hasil Perikanan 03, 199–206.
Wu, T., Wang, M., Wang, P., Tian, H., & Zhan, P. (2022). Advances in the Formation and Control Methods of Undesirable Flavors in Fish. Foods, 11(16). https://doi.org/10.3390/foods11162504
Yulia, D.N., Maherawati, S. P. (2024). Formulation of Sago Crackers With the Addition of. 9(2), 126–132.
Zhang, M., Liu, Y., Jin, M., Li, D., Wang, Z., Jiang, P., & Zhou, D. (2023). The Effect of Heat Treatment on the Digestion and Absorption Properties of Protein in Sea Cucumber Body Wall. Foods, 12(15) 1–15. https://doi.org/10.3390/foods12152896
Zhang, X., Min Zhang, & Adhikari, B. (2020). Recent developments in frying technologies applied to fresh foods. Trends in Food Science & Technology, 98, 68–81.
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